Rapid diagnosis of respiratory syncytial virus infection by antigen immunofluorescence detection with monoclonal antibodies and immunoglobulin M immunofluorescence test
نویسندگان
چکیده
منابع مشابه
Rapid detection of respiratory syncytial virus by indirect immunofluorescence in infants hospitalized with acute respiratory disease.
Respiratory syncytial virus (RSV) is well recognized as the single most important pathogen accounting for acute viral infections of lower respiratory tract in infants and young children. Outbreaks of RSV infections occur each year in winter and early spring (L Pednealut et al. 1994 J Clin Microbiol 32: 2861-2864). In Cuba, acute respiratory diseases (ARD) constitute a major problem of health, a...
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The detection of immunoglobulin M (IgM) antibodies to cytomegalovirus-induced late antigens by an indirect immunofluorescence assay was improved by the use of monoclonal antibodies to human IgM. Nonspecific background fluorescence was absent, facilitating the reading of the slides and the detection of a specific fluorescence reaction in sera with low levels of specific IgM. Moreover, the indire...
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A simple method is presented for producing large numbers of inclusions of Chlamydia trachomatis serotype L2 in cell cultures on slides for immunofluorescence antibody staining. Preliminary results with a total of 1,241 human sera from different groups were consistent with findings by earlier methods: 82% of chlamydia-positive men with nongonococcal urethritis had antibodies at titers of greater...
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Seventy-eight unselected children under the age of 2 years suffering from acute respiratory infections were investigated by the fluorescent antibody technique and a comparison was made with conventional isolation and serological methods. Sixty-nine per cent. of children with bronchiolitis were diagnosed as suffering from respiratory syncytial virus infection on the day of admission by examinati...
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The use of fluorescein-conjugated antiserum against respiratory syncytial (RS) and parainfluenza 1 and 3 viruses was compared with conventional techniques in the rapid detection of virus in tissue cultures inoculated with pharyngeal specimens known to contain these viruses. Twenty-three specimens were tested: 9 RS, 8 parainfluenza 1, and 6 parainfluenza 3. The fluorescent-antibody technique (FA...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1986
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.24.6.1038-1040.1986